Phenyl isothiocyanate 103-72-0 PITC

The R1 cell line was established in August 1991 and was derived from 3.5-day blastocysts obtained through mating of two 129 sublines (129S1/SvImJand129X1/SvJ). The pluripotency of R1 was initially detected through tetraploid embryo testing, and of course, diploid embryo testing can also be used. However, when tetraploid aggregates were tested, approximately 20% of the subclones from the 14th generation had the original developmental potential of R1. Animals derived from R1 are fertile when they reach adulthood. Injecting genetically modified R1 cells into C57 blastocysts or aggregating them with CD-1 or distant inbred ICR8 cell-stage embryos can produce efficient germline delivery. More than 90% of knockout clones enter the germline through aggregation chimeras (n>60). Each batch passed the mycoplasma test in our library and the result was negative. When culturing, Kunming White MEF (SCSP-101R) should be used as the feeder layer cells. It is P24 generation when frozen.